European Concerted Action on Anticoagulation (ECAA). An assessment of lyophilised plasmas for ISI calibration of CoaguChek and TAS whole blood prothrombin time monitors

被引：13

作者：

Poller, L ^{[1
]}

Keown, M

Chauhan, N

van den Besselaar, AMHP

Tripodi, A

Shiach, C

Jespersen, J

机构：

[1] Univ Manchester, Sch Biol Sci, ECAA Cent Facil, Manchester M13 9PT, Lancs, England

[2] Leiden Univ, Med Ctr, Haemostasis & Thrombosis Res Ctr, NL-2333 ZA Leiden, Netherlands

[3] Univ Milan, Maggiore Hosp, IRCCS, Haemophilia & Thrombosis Res, I-20122 Milan, Italy

[4] Manchester Royal Infirm, Dept Haematol, Manchester M13 9WL, Lancs, England

[5] Univ So Denmark, Dept Thrombosis Res, DK-6800 Esbjerg, Denmark

[6] Ribe Cty Hosp, Dept Clin Biochem, DK-6800 Esbjerg, Denmark

来源：

JOURNAL OF CLINICAL PATHOLOGY | 2003年 / 56卷 / 02期

关键词：

D O I：

10.1136/jcp.56.2.114

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Aims: The recommended method for the international sensitivity index (ISI) calibration of whole blood point of care testing (POCT) prothrombin time (PT) systems was originally described by Tripodi et al in 1993 but is too complex and demanding. The present European Concerted Action on Anticoagulation (ECAA) study aimed to assess the reliability of simpler ISI calibration using lyophilised plasma samples. Methods: ISI calibrations using three different types of ECAA lyophilised plasma samples (artificially depleted, individual, and pooled coumarin) were compared with whole blood calibrations on CoaguChek Mini and TAS PT-NC POCT monitors at 10 centres. Results: With CoaguChek Mini systems, lyophilised coumarin plasma samples (both single donation and pooled) gave ISI and international normalised ratio (INR) values comparable to whole blood. With artificially depleted plasma, ISI and INR values were too high. With TAS PT-NC systems, all three types of lyophilised plasma samples gave inaccurate ISI and unreliable INR results, similar to previous ECAA findings with fresh plasma calibrations. Conclusions: With CoaguChek Mini systems, ISI calibration can be simplified by the use of ECAA lyophilised plasma samples from coumarin treated patients. Further study is needed to devise a simpler calibration method for the TAS PT-NC system.

引用

页码：114 / 119

页数：6

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