Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass spectrometry

被引：99

作者：

Strelkov, S ^{[1
]}

von Elstermann, M ^{[1
]}

Schomburg, D ^{[1
]}

机构：

[1] Univ Cologne, Inst Biochem, D-50674 Cologne, Germany

来源：

BIOLOGICAL CHEMISTRY | 2004年 / 385卷 / 09期

关键词：

Corynebacterium glutamicum; gas chromatography; mass spectrometry; metabolic profiling; metabolite; metabolome; metabolomics;

D O I：

10.1515/BC.2004.111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical method based on gas chromatography/ mass spectrometry was developed for metabolome investigation of Corynebacterium glutamicum. For the first time a fast method for metabolic screening that can be automated is described for this organism. More than 1000 compounds could be detected per experiment, ca. 330 of those showed a peak area significantly above background. Out of these 164 compounds were identified so far, representing derivatives of 121 different metabolites, which were quantified in one sample. In spite of the different chemical nature of metabolites and high matrix content, a measurement reproducibility in the range of 6% error was achieved. The application of this method for the analysis of the adaptation of C. glutamicum to different growth conditions is demonstrated.

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收藏

页码：853 / 861

页数：9

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