Stereoselective prostereogenic 3-oxo ester reduction mediated by a novel yeast alcohol dehydrogenase derived from Kluyveromyces marxianus CBS 6556

被引：12

作者：

Perrone, Maria Grazia

Santandrea, Ernesto

Scilimati, Antonio

Syldatk, Christoph

机构：

[1] Univ Bari, Dipartimento Farmacochim, I-70125 Bari, Italy

[2] Univ Karlsruhe, Engler Bunte Inst, Lehrstuhl Tech Biol, D-76131 Karlsruhe, Germany

来源：

ADVANCED SYNTHESIS & CATALYSIS | 2007年 / 349卷 / 07期

关键词：

alcohol dehydrogenase; asymmetric bioreduction; fermentation; Kluyveromyces marxianus; 3-oxo esters;

D O I：

10.1002/adsc.200600379

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A yeast alcohol dehydrogenase (ADH) has been purified up to a purification factor value of 21,731-fold from Kluyveromyces marxianus CBS 6556. The purification procedure consisted of two chromatographic steps (DEAE-anion exchange and affinity chromatography). The optimal pH was 7, its optimal temperature was 40 degrees C and its co-factor was NADPH. This novel ADH efficiently mediated the reduction of 3-oxo esters with a high degree of ste-reoselectivity, providing chiral alcohols having the (S) absolute configuration at the newly formed stereogenic centre by delivering the hydride from the re-face of the prochiral carbonyl compounds.

引用

页码：1111 / 1118

页数：8

共 29 条

[1] SIR97:: a new tool for crystal structure determination and refinement [J].