Reverse transcription-booster PCR for detection of noroviruses in shellfish

被引：26

作者：

De Medici, D ^{[1
]}

Croci, L ^{[1
]}

Suffredini, E ^{[1
]}

Toti, L ^{[1
]}

机构：

[1] Ist Super Sanita, Lab Alimenti, I-00161 Rome, Italy

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 10期

关键词：

D O I：

10.1128/AEM.70.10.6329-6332.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RTPCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the NY strains tested compared with the standard RT-PCR method. Higher sensitivity was confirmed in tests on experimentally and naturally contaminated shellfish.

引用

收藏

页码：6329 / 6332

页数：4

相关论文

共 20 条

[1] INSTANT RNA ISOLATION FROM VIRUS-INFECTED TISSUE-CULTURE FLUID FOR THE POLYMERASE CHAIN-REACTION [J].

AFZAL, MA ;

MINOR, PD .

VACCINE, 1994, 12 (11) :976-977

[2]

Ang L H, 1998, Commun Dis Public Health, V1, P38

[3] Diagnosis of noncultivatable gastroenteritis viruses, the human caliciviruses [J].

Atmar, RL ;

Estes, MK .

CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (01) :15-+

[4] ETIOLOGY OF CAT-SCRATCH DISEASE - COMPARISON OF POLYMERASE CHAIN-REACTION DETECTION OF BARTONELLA (FORMERLY ROCHALIMAEA) AND AFIPIA-FELIS DNA WITH SEROLOGY AND SKIN-TESTS [J].

BERGMANS, AMC ;

GROOTHEDDE, JW ;

SCHELLEKENS, JFP ;

VANEMBDEN, JDA ;

OSSEWAARDE, JM ;

SCHOULS, LM .

JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (04) :916-923

[5] Waterborne outbreak of Norwalk-like virus gastroenteritis at a tourist resort, Italy [J].

Boccia, D ;

Tozzi, AE ;

Cotter, B ;

Rizzo, C ;

Russo, T ;

Buttinelli, G ;

Caprioli, A ;

Marziano, ML ;

Ruggeri, FM .

EMERGING INFECTIOUS DISEASES, 2002, 8 (06) :563-568

[6] DETECTION OF SALMONELLA-ENTERITIDIS IN FECES FROM POULTRY USING BOOSTER POLYMERASE CHAIN-REACTION AND OLIGONUCLEOTIDE PRIMERS SPECIFIC FOR ALL MEMBERS OF THE GENUS SALMONELLA [J].

COHEN, ND ;

MCGRUDER, ED ;

NEIBERGS, HL ;

BEHLE, RW ;

WALLIS, DE ;

HARGIS, BM .

POULTRY SCIENCE, 1994, 73 (02) :354-357

[7] Detection of hepatitis A virus in shellfish by nested reverse transcription-PCR [J].

Croci, L ;

De Medici, D ;

Morace, G ;

Fiore, A ;

Scalfaro, C ;

Beneduce, F ;

Toti, L .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 48 (01) :67-71

[8] Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCR [J].

De Medici, D ;

Croci, L ;

Di Pasquale, S ;

Fiore, A ;

Toti, L .

LETTERS IN APPLIED MICROBIOLOGY, 2001, 33 (05) :362-366

[9]

GREEN SM, 1994, J GEN VIROL, V75, P3

[10] Evaluation of nine sets of PCR primers in the RNA dependent RNA polymerase region for detection and differentiation of members of the family Caliciviridae, Norwalk virus and Sapporo virus [J].

Honma, S ;

Nakata, S ;

Kinoshita-Numata, K ;

Kogawa, K ;

Chiba, S .

MICROBIOLOGY AND IMMUNOLOGY, 2000, 44 (05) :411-419