Multiphoton endoscopy

被引：252

作者：

Jung, JC

Schnitzer, MJ

机构：

[1] Bell Labs, Lucent Technol, Murray Hill, NJ 07974 USA

[2] Univ Oxford, Dept Pharmacol, Oxford OX1 3QT, England

来源：

OPTICS LETTERS | 2003年 / 28卷 / 11期

关键词：

D O I：

10.1364/OL.28.000902

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Despite widespread use of multiphoton fluorescence microscopy, development of endoscopes for nonlinear optical imaging has been stymied by the degradation of ultrashort excitation pulses that occurs within optical fiber as a result of the combined effects of group-velocity dispersion and self-phase modulation. We introduce microendoscopes (350-1000 mum in diameter) based on gradient-index microlenses that effectively eliminate self-phase modulation within the endoscope. Laser-scanning multiphoton fluorescence endoscopy exhibits micrometer-scale resolution. We used multiphoton endoscopes to image fluorescently labeled neurons and dendrites. (C) 2003 Optical Society of America.

引用

页码：902 / 904

页数：3

共 19 条

[1] Compact two-photon fluorescence microscope based on a single-mode fiber coupler [J].