Stimulation of interleukin-6 release by interleukin-1β from isolated human adipocytes

被引:60
作者
Flower, L
Gray, R
Pinkney, J
Mohamed-Ali, V
机构
[1] Royal Free & UCL, Sch Med, Dept Med, Adipokines & Metab Res Grp, London W1W 7EY, England
[2] Aintree Univ Hosp NHS Fdn Trust, Ctr Clin Sci, Dept Med, Diabet & Endocrinol Res Grp, Liverpool L9 7AL, Merseyside, England
关键词
adipocyte; peripheral blood cells; cytokines; adipokines; interleukin-6; interleukin-1; beta;
D O I
10.1016/S1043-4666(02)00495-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The secretion of interleukin-6 (IL-6) is modulated by immune, hormonal and metabolic stimuli in a cell-specific manner. We investigated the effect of cytokines, TNFalpha and IL-1beta, and insulin on IL-6 release from human adipocytes and peripheral blood cells (PBC). Adipocytes released IL-6 constitutively (after 5h: 5.64 [1.61-15.30]pg ml(-1), after 10h: 15.95 [2.34-45.59]pg ml(-1), p = 0.007), while PBC secretion did not change significantly over this period. LPS stimulated IL-6 secretion in PBC after 5 h but was without effect on adipocytes. TNFalpha and insulin induced IL-6 production from PBC, but had no effect on adipocytes. IL-1beta, however, induced a substantial increase in IL-6 release in adipocytes and PBC (all p < 0.05). Adipose tissue production of IL-1beta was assessed in vivo by measuring arterio-venous differences across the subcutaneous abdominal adipose bed. Net release of IL-1beta was not observed, suggesting that under basal conditions there is no detectable release of this cytokine into the circulation from this depot. In conclusion (1) PBC demonstrate regulated IL-6 release, while the adipocyte release has a large constitutive component; (2) immune modulators, such as LPS, TNFalpha and IL-1beta, all induce PBC IL-6 release, but only IL-1beta stimulates adipocyte release. Though IL-1beta is not an endocrine signal from adipose tissue, it is an autocrine/paracrine stimulator of IL-6 release from human adipocytes. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:32 / 37
页数:6
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