Preparation of recombinant human GDNF by baculovirus expression system and analysis of its biological activities

被引：10

作者：

Chen, ZY

Sun, JX

Li, JH

He, C

Lu, CL

Wu, XF

机构：

[1] Second Mil Med Univ, Dept Neurobiol, Shanghai 200433, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 273卷 / 03期

关键词：

GDNF; baculovirus; purification; dopaminergic neuron; regeneration;

D O I：

10.1006/bbrc.2000.3046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant human glial cell line-derived neurotrophic factor (GDNF) has been expressed at high levels and produced in large quantities in baculovirus-infected Trichoplusia ni cells (Tn-5B1-4). The glycosylated protein was purified using immunoaffinity chromatography and gel filtration. Pure, recombinant human GDNF promoted the survival and morphological differentiation of embryonic dopaminergic neurons and enhanced axonal regeneration after sciatic nerve transection. Because recombinant bioactive human GDNF can be obtained in large quantities, and purified to near homogeneity, they are suitable for evaluation in animal models. (C) 2000 Academic Press.

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收藏

页码：902 / 906

页数：5

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