Use of pyrosequencing to identify point mutations in domain V of 23S rRNA genes of linezolid-resistant Staphylococcus aureus and Staphylococcus epidermidis

被引：22

作者：

Zhu, W.

Tenover, F. C.

Limor, J.

Lonsway, D.

Prince, D.

Dunne, W. M., Jr.

Patel, J. B.

机构：

[1] Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA 30333 USA

[2] Ctr Dis Control & Prevent, Sci Resources Program, Atlanta, GA 30333 USA

[3] Piedmont Hosp, Microbiol Lab, Atlanta, GA 30309 USA

[4] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2007年 / 26卷 / 03期

关键词：

D O I：

10.1007/s10096-007-0261-0

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A pyrosequencing assay was used for the rapid characterization of linezolid-resistant isolates of Staphylococcus aureus and Staphylococcus epidermidis. The assay identified base substitutions in copies of the 23S rRNA gene and determined the percentage of alleles with the mutation. Modifications of the assay were necessary to identify all mutations in the 23S rRNA genes of S. epidermidis that were associated with linezolid resistance. A C2534T mutation was identified in S. epidermidis that was not previously reported in a linezolid-resistant isolate.

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页码：161 / 165

页数：5

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