Apical surface formation in MDCK cells:: regulation by the serine/threonine kinase EMK1

被引：24

作者：

Cohen, D ^{[1
]}

Müsch, A ^{[1
]}

机构：

[1] Cornell Univ, Weill Med Coll, M Dyson Inst Vis Res, New York, NY 10021 USA

来源：

METHODS | 2003年 / 30卷 / 03期

关键词：

D O I：

10.1016/S1046-2023(03)00033-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

It has recently become evident that basic mechanisms for the establishment of cell polarity are conserved between epithelial and nonepithelial systems. The vast catalogue of known gene products involved in various aspects of invertebrate and yeast cell polarity provides a repertoire of candidate proteins that can be tested for their roles in the organization of mammalian epithelia. Here, we describe cell biological approaches to study the development and maintenance of cell polarity in Mardin-Darby canine kidney (MDCK) cells, an established mammalian model cell line for simple epithelia. The assays allowed us to characterize the Caenorhabditis elegans PAR-1 homologue EMK1 as a novel regulator of apical surface formation in epithelial cells. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：269 / 276

页数：8

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