Aptamer-DNAzyme Hairpins for Amplified Biosensing

被引:182
作者
Teller, Carsten [1 ]
Shimron, Simcha [1 ]
Willner, Itamar [1 ]
机构
[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel
基金
以色列科学基金会;
关键词
FUNCTIONALIZED AU NANOPARTICLES; MOLECULAR BEACONS; RNA APTAMERS; DNA APTAMER; ELECTROCHEMICAL DETECTION; CATALYTIC LABELS; SELECTION; SENSORS; ADENOSINE; ACTIVATION;
D O I
10.1021/ac901773b
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Engineered nucleic acid hairpin structures are used for the amplified analysis of low-molecular-weight substrates (adenosine monophosphate, AMP) or proteins (lysozyme). The hairpin structures consist of the anti-AMP or antilysozyme aptamer units linked to the horseradish peroxidase (HRP)-mimicking DNAzyme sequence. The HRP-mimicking DNAzyme sequence is protected in a "caged", inactive structure in the stem regions of the respective hairpins, whereas the loop regions include a part of the respective aptamer sequence. The opening of the hairpins by the analytes, AMP or lysozyme, through the formation of the respective analyte-aptamer complexes, results in the self-assembly of the active HRP-mimicking DNAzyme. The DNAzyme catalyzes the H2O2-mediated oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(2)-) to the colored ABTS(center dot-), thus providing the amplified optical detection of the respective analytes. The engineered aptamer-DNAzyme hairpin structures reveal significantly improved analytical performance, as compared to analogous fluorophore-quencher-labeled hairpins.
引用
收藏
页码:9114 / 9119
页数:6
相关论文
共 50 条
[1]  
Baker BR, 2006, J AM CHEM SOC, V128, P3138, DOI 10.1021/ja056957p
[2]   ISOLATION OF RNA APTAMERS FOR BIOLOGICAL COFACTORS BY IN-VITRO SELECTION [J].
BURGSTALLER, P ;
FAMULOK, M .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1994, 33 (10) :1084-1087
[3]   Different approaches for the detection of thrombin by an electrochemical aptamer-based assay coupled to magnetic beads [J].
Centi, S. ;
Messina, G. ;
Tombelli, S. ;
Palchetti, I. ;
Mascini, M. .
BIOSENSORS & BIOELECTRONICS, 2008, 23 (11) :1602-1609
[4]   Automated selection of anti-protein aptamers [J].
Cox, JC ;
Ellington, AD .
BIOORGANIC & MEDICINAL CHEMISTRY, 2001, 9 (10) :2525-2531
[5]   Quantum dot-antibody and aptamer conjugates shift fluorescence upon binding bacteria [J].
Dwarakanath, S ;
Bruno, JG ;
Shastry, A ;
Phillips, T ;
John, A ;
Kumar, A ;
Stephenson, LD .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 325 (03) :739-743
[6]   Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: Applications for biosensing and logic gate operations [J].
Elbaz, Johann ;
Shlyahovsky, Bella ;
Li, Di ;
Willner, Itamar .
CHEMBIOCHEM, 2008, 9 (02) :232-239
[7]   Switchable Motion of DNA on Solid Supports [J].
Elbaz, Johann ;
Tel-Vered, Ran ;
Freeman, Ronit ;
Yildiz, Huseyin Bekir ;
Willner, Itamar .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (01) :133-137
[8]   Coherent Activation of DNA Tweezers: A "SET-RESET" Logic System [J].
Elbaz, Johann ;
Moshe, Michal ;
Willner, Itamar .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (21) :3834-3837
[9]   Cooperative Multicomponent Self-Assembly of Nucleic Acid Structures for the Activation of DNAzyme Cascades: A Paradigm for DNA Sensors and Aptasensors [J].
Elbaz, Johann ;
Moshe, Michal ;
Shlyahovsky, Bella ;
Willner, Itamar .
CHEMISTRY-A EUROPEAN JOURNAL, 2009, 15 (14) :3411-3418
[10]   INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].
ELLINGTON, AD ;
SZOSTAK, JW .
NATURE, 1990, 346 (6287) :818-822