Single-molecule spectroscopy for nucleic acid analysis: A new approach for disease detection and genomic analysis

被引：6

作者：

Goodwin, PM ^{[1
]}

Nolan, RL ^{[1
]}

Cai, H ^{[1
]}

机构：

[1] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA

来源：

CURRENT PHARMACEUTICAL BIOTECHNOLOGY | 2004年 / 5卷 / 03期

关键词：

single-molecule; fluorescence; two-color; haplotyping; gene expression;

D O I：

10.2174/1389201043376814

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently developed single-molecule spectroscopy (SMS) permits the analysis of fluorescent mixtures one molecule at a time. SMS methods provide the means to make rapid measurements on small, complex samples without the need for separations and target amplification enabling a new class of ultrasensitive nucleic acid assays. Here we give a brief overview of the current state of the art of SMS nucleic acid analysis and discuss ongoing work in our laboratory on two-color single-molecule fluorescence detection of specific nucleic acid sequences. In the future, two-color SMS nucleic acid assays will be used for a variety of applications including: gene expression analysis, disease detection and genomics.

引用

页码：271 / 278

页数：8

共 63 条

[1] Reduction of luminescent background in ultrasensitive fluorescence detection by photobleaching [J].

Affleck, RL ;

Ambrose, WP ;

Demas, JN ;

Goodwin, PM ;

Schecker, JA ;

Wu, JM ;

Keller, RA .

ANALYTICAL CHEMISTRY, 1996, 68 (13) :2270-2276

[2] Single molecule fluorescence spectroscopy at ambient temperature [J].

Ambrose, WP ;

Goodwin, PM ;

Jett, JH ;

Van Orden, A ;

Werner, JH ;

Keller, RA .

CHEMICAL REVIEWS, 1999, 99 (10) :2929-2956

[3]

AMBROSE WP, 2003, TOPICS FLUORESCENCE, V7, P239

[4] Strategies for signal amplification in nucleic acid detection [J].

Andras, SC ;

Power, JB ;

Cocking, EC ;

Davey, MR .

MOLECULAR BIOTECHNOLOGY, 2001, 19 (01) :29-44

[5] Optical mapping and its potential for large-scale sequencing projects [J].

Aston, C ;

Mishra, B ;

Schwartz, DC .

TRENDS IN BIOTECHNOLOGY, 1999, 17 (07) :297-302

[6] PCR AMPLIFICATION OF UP TO 35-KB DNA WITH HIGH-FIDELITY AND HIGH-YIELD FROM LAMBDA-BACTERIOPHAGE TEMPLATES [J].

BARNES, WM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2216-2220

[7] A new vector for recombination-based cloning of large DNA fragments from yeast artificial chromosomes [J].

Bradshaw, MS ;

Bollekens, JA ;

Ruddle, FH .

NUCLEIC ACIDS RESEARCH, 1995, 23 (23) :4850-4856

[8] Sequence information can be obtained from single DNA molecules [J].

Braslavsky, I ;

Hebert, B ;

Kartalov, E ;

Quake, SR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :3960-3964

[9] Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems [J].

Bustin, SA .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2002, 29 (01) :23-39

[10] Ultrasensitive, direct detection of a specific DNA sequence of Bacillus anthracis in solution [J].

Castro, A ;

Okinaka, RT .

ANALYST, 2000, 125 (01) :9-11

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