Self-Controlled Monofunctionalization of Quantum Dots for Multiplexed Protein Tracking in Live Cells

被引：58

作者：

You, Changjiang ^{[1
]}

Wilmes, Stephan ^{[1
]}

Beutel, Oliver ^{[1
]}

Loechte, Sara ^{[1
]}

Podoplelowa, Yulia ^{[1
]}

Roder, Friedrich ^{[1
]}

Richter, Christian ^{[1
]}

Seine, Thomas ^{[1
]}

Schaible, Dirk ^{[1
]}

Uze, Gilles ^{[2
]}

Clarke, Samuel ^{[3
]}

Pinaud, Fabien ^{[3
]}

Dahan, Maxime ^{[3
]}

Piehler, Jacob ^{[1
]}

机构：

[1] Univ Osnabruck, Div Biophys, D-49076 Osnabruck, Germany

[2] Univ Montpellier 2, CNRS, UMR 5124, Montpellier, France

[3] Ecole Normale Super, Dept Phys & Biol, F-75231 Paris, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 24期

关键词：

fluorescence microscopy; nanoparticles; protein-protein interactions; quantum dots; receptors; HISTIDINE-TAGGED PROTEINS; LIVING CELLS; RECEPTORS; MONOVALENT; DYNAMICS; LIGANDS; SITE;

D O I：

10.1002/anie.200907032

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Functionalization of quantum dots (QDs) was achieved by controlling the surface density of functional groups by electrostatic repulsion. These QDs were conjugated with His-tagged proteins in vitro and in live cells by self-assembly without requiring further fractionation, and dualcolor tracking of cell surface receptors was possible. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：4108 / 4112

页数：5

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