Construction of Injectable Double-Network Hydrogels for Cell Delivery

被引：79

作者：

Yan, Yan ^{[1
]}

Li, Mengnan ^{[2
]}

Yang, Di ^{[1
]}

Wang, Qian ^{[2
]}

Liang, Fuxin ^{[2
]}

Qu, Xiaozhong ^{[1
]}

Qiu, Dong ^{[2
]}

Yang, Zhenzhong ^{[2
]}

机构：

[1] Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing 100049, Peoples R China

[2] Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China

来源：

BIOMACROMOLECULES | 2017年 / 18卷 / 07期

基金：

中国国家自然科学基金;

关键词：

IN-SITU; CROSS-LINKING; BIOMEDICAL APPLICATIONS; DRUG-DELIVERY; STEM-CELLS; CARTILAGE; ALGINATE; CHITOSAN; TOUGH; REGENERATION;

D O I：

10.1021/acs.biomac.7b00452

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Herein we present a unique method of using dynamic cross-links, which are dynamic covalent bonding and ionic interaction, for the construction of injectable double-network (DN) hydrogels, with the objective of cell delivery for cartilage repair. Glycol chitosan and dibenzaldhyde capped poly(ethylene oxide) formed the first network, while calcium alginate formed the second one, and in the resultant DN hydrogel, either of the networks could be selectively removed. The moduli of the DN hydrogel were significantly improved compared to that of the parent single-network hydrogels and were tunable by changing the chemical components. In situ 3D cell encapsulation could be easily performed by mixing cell suspension to the polymer solutions and transferred through a syringe needle before sol gel transition. Cell proliferation and mediated differentiation of mouse chondrogenic cells were achieved in the DN hydrogel extracellular matrix.

引用

页码：2128 / 2138

页数：11

共 55 条

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THE CHONDROCYTE, ARCHITECT OF CARTILAGE - BIOMECHANICS, STRUCTURE, FUNCTION AND MOLECULAR-BIOLOGY OF CARTILAGE MATRIX MACROMOLECULES [J].