The 3′ termini of mouse Piwi-interacting RNAs are 2′-O-methylated

被引：172

作者：

Ohara, Tomoya

Sakaguchi, Yuriko

Suzuki, Takeo

Ueda, Hiroki

Miyauchi, Kenjyo

Suzuki, Tsutomu

机构：

[1] Univ Tokyo, Dept Chem & Biotechnol, Grad Sch Engn, Bunkyo Ku, Tokyo 138656, Japan

[2] Japan Biol Informat Consortium, Koto Ku, Tokyo 1350064, Japan

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2007年 / 14卷 / 04期

关键词：

D O I：

10.1038/nsmb1220

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Piwi-interacting RNAs (piRNAs) are a germline-specific class of small noncoding RNAs that are essential for spermatogenesis, but their function and biogenesis remain elusive. Here we report a post-transcriptional modification of mouse piRNAs. Mass spectrometric analysis reveals that the piRNAs tested are fully modified by 2'-O-methylation at their 3' termini. This observation may provide a clue to the biogenesis and function of piRNAs in spermatogenesis.

引用

收藏

页码：349 / 350

页数：2

相关论文

共 16 条

[1] A novel class of small RNAs bind to MILI protein in mouse testes [J].

Aravin, Alexei ;

Gaidatzis, Dimos ;

Pfeffer, Sebastien ;

Lagos-Quintana, Mariana ;

Landgraf, Pablo ;

Iovino, Nicola ;

Morris, Patricia ;

Brownstein, Michael J. ;

Kuramochi-Miyagawa, Satomi ;

Nakano, Toru ;

Chien, Minchen ;

Russo, James J. ;

Ju, Jingyue ;

Sheridan, Robert ;

Sander, Chris ;

Zavolan, Mihaela ;

Tuschl, Thomas .

NATURE, 2006, 442 (7099) :203-207

[2] ULTRASONICALLY ASSISTED ELECTROSPRAY-IONIZATION FOR LC/MS DETERMINATION OF NUCLEOSIDES FROM A TRANSFER-RNA DIGEST [J].

BANKS, JF ;

SHEN, S ;

WHITEHOUSE, CM ;

FENN, JB .

ANALYTICAL CHEMISTRY, 1994, 66 (03) :406-414

[3] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[4] Extensive 3′ modification of plant small RNAs is modulated by helper component-proteinase expression [J].

Ebhardt, HA ;

Thi, EP ;

Wang, MB ;

Unrau, PJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (38) :13398-13403

[5] Post-transcriptional gene silencing by siRNAs and miRNAs [J].

Filipowicz, W ;

Jaskiewicz, L ;

Kolb, FA ;

Pillai, RS .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2005, 15 (03) :331-341

[6] A germline-specific class of small RNAs binds mammalian Piwi proteins [J].

Girard, Angelique ;

Sachidanandam, Ravi ;

Hannon, Gregory J. ;

Carmell, Michelle A. .

NATURE, 2006, 442 (7099) :199-202

[7] A novel class of small RNAs in mouse spermatogenic cells [J].

Grivna, Shane T. ;

Beyret, Ergin ;

Wang, Zhong ;

Lin, Haifan .

GENES & DEVELOPMENT, 2006, 20 (13) :1709-1714

[8] Small RNAs just got bigger: Piwi-interacting RNAs (piRNAs) in mammalian testes [J].

Kim, V. Narry .

GENES & DEVELOPMENT, 2006, 20 (15) :1993-1997

[9]

KIRINO T, 2007, NAT STRUCT MOL 0325

[10] Characterization of the piRNA complex from rat testes [J].

Lau, Nelson C. ;

Seto, Anita G. ;

Kim, Jinkuk ;

Kuramochi-Miyagawa, Satomi ;

Nakano, Toru ;

Bartel, David P. ;

Kingston, Robert E. .

SCIENCE, 2006, 313 (5785) :363-367