A portable device for temperature control along microchannels

被引:39
作者
Vigolo, Daniele [1 ,2 ]
Rusconi, Roberto [2 ]
Piazza, Roberto [1 ]
Stone, Howard A. [2 ]
机构
[1] Politecn Milan, Dipartimento Chim Mat & Ingn Chim, I-20133 Milan, Italy
[2] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
基金
美国国家科学基金会;
关键词
MICROFLUIDIC DEVICES; POLY(DIMETHYLSILOXANE); AMPLIFICATION; FABRICATION; CHIP; DNA; PCR;
D O I
10.1039/b919146a
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
For many physical, chemical and biological measurements, temperature is a crucial parameter to control. In particular, the recent development of microreactors and chip-based technologies requires integrated thermostatic systems. However, the requirements of disposability and visual inspection of a device under a microscope cannot accommodate equipment such as external heaters. By exploiting a silver-filled epoxy that can be injected and solidified in a microfluidic chip, we demonstrate a simple and inexpensive design of a conductive path, which allows heating by the Joule effect of both sides of a microchannel. In addition to permitting the maintenance of a constant temperature along the channel walls, our method can control the temperature gradient across the channel, thus enabling non-equilibrium studies in a microfluidic geometry.
引用
收藏
页码:795 / 798
页数:4
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