Detection of Hybridization on Nanografted Oligonucleotides Using Scanning Near-Field Infrared Microscopy

被引：14

作者：

Kopf, Ilona ^{[1
]}

Grunwald, Christian ^{[2
,3
]}

Bruendermann, Erik ^{[1
]}

Casalis, Loredana ^{[2
,4
]}

Scoles, Giacinto ^{[2
,3
,4
]}

Havenith, Martina ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Bochum, Germany

[2] ELETTRA, Sincrotrone Trieste SCpA, Trieste, Italy

[3] SISSA, I-34014 Trieste, Italy

[4] IIT SISSA Unit, Trieste, Italy

来源：

JOURNAL OF PHYSICAL CHEMISTRY C | 2010年 / 114卷 / 02期

关键词：

SECONDARY STRUCTURE; DNA HYBRIDIZATION; GOLD SURFACES; NANOSTRUCTURES; MONOLAYERS; KINETICS; SPECTROSCOPY; RESOLUTION; SNIM;

D O I：

10.1021/jp906813f

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We demonstrate that scattering scanning near-field infrared microscopy (s-SNIM) is a label free analytical method allowing hybridization detection of nanografted DNA patches on a sub-mu M scale. Oil the basis of their distinct dielectric properties in the IR. we can distinguish between single stranded and double stranded DNA. The sensitivity of s-SNIM is found to be increased by 7 orders of magnitude compared to conventional FTIR spectroscopy due to the tip enhanced interaction with the Substrate.

引用

收藏

页码：1306 / 1311

页数：6

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