Sensor and biosensor based on Prussian Blue modified gold and platinum screen printed electrodes

被引:114
作者
de Mattos, IL [1 ]
Gorton, L [1 ]
Ruzgas, T [1 ]
机构
[1] Lund Univ, Dept Analyt Chem, S-22100 Lund, Sweden
基金
巴西圣保罗研究基金会;
关键词
Prussian Blue; screen-printed electrodes; amperometric sensor; biosensor;
D O I
10.1016/S0956-5663(02)00185-9
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Gold (An) and platinum (Pt) screen-printed electrodes were modified with Prussian Blue (PB) for the development of amperometric sensors selective for hydrogen peroxide detection. The sensors exhibited sensitivities towards H2O2 equal to 2 A M-1 cm(-2) for An and 1 A M-1 cm(-2) for Pt electrodes. The sensors were also employed as the basis for construction of glucose biosensors through further modification with crystallised glucose oxidase immobilised in a Nafion membrane. In order to improve the operational stability of the modified electrodes a buffer solution containing tetrabutylammonium toluene-4-sulfonate was used. The long-term performance of the sensors and biosensors were evaluated by continuous monitoring of hydrogen peroxide and glucose solutions (50 muM and 1 mM, respectively) in the flow-injection mode for 10 h. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:193 / 200
页数:8
相关论文
共 37 条