Ratiometric pH-dot ANSors

被引：37

作者：

Wang, Xiaojuan ^{[1
]}

Boschetti, Chiara ^{[1
]}

Ruedas-Rama, Maria Jose ^{[1
]}

Tunnacliffe, Alan ^{[1
]}

Hall, Elizabeth A. H. ^{[1
]}

机构：

[1] Univ Cambridge, Inst Biotechnol, Dept Chem Engn & Biotechnol, Cambridge CB2 1QT, England

来源：

ANALYST | 2010年 / 135卷 / 07期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

CDSE QUANTUM DOTS; RANGE; PROBE; NANOPARTICLES; NANOSPHERES; NANOSENSORS; PARTICLES; OXYGEN; CELLS;

D O I：

10.1039/b922751b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A silica based analytical nanosphere sensor (ANSor) containing quantum dots (QDs) is reported, which can measure local pH in a ratiometric fashion. A silane modified reference QD was incorporated into a silica matrix by the Stober method hydrolysis and polycondensation of tetraethoxysilane, giving highly fluorescent QD-embedded silica particles with high yield. A further QD was then bonded onto the silica particle surface and modified with Nile Blue to render it pH responsive. These two populations of QDs were excited simultaneously and gave out well-separated emission peaks which could be taken as a ratio to yield a ratiometric estimate of pH. The sensors are stable, robust and capable of measuring pH in the physiologically relevant range.

引用

页码：1585 / 1591

页数：7

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