Femtosecond laser-induced crystallization of protein in gel medium

被引：40

作者：

Nakamura, Kazuhiko

Sora, Yosuke

Yoshikawa, Hiroshi Y.

Hosokawa, Yoichiroh

Murai, Ryota

Adachi, Hiroaki

Mori, Yusuke

Sasaki, Takatomo

Masuhara, Hiroshi

机构：

[1] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Dept Elect Engn, Suita, Osaka 5650871, Japan

[4] CREST, JST, Suita, Osaka 5650871, Japan

来源：

APPLIED SURFACE SCIENCE | 2007年 / 253卷 / 15期

关键词：

femtosecond laser; laser ablation; light-induced crystallization; protein crystal; hen egg-white lysozyme;

D O I：

10.1016/j.apsusc.2007.01.046

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We succeeded in generating femtosecond laser-induced crystallization of hen egg-white lysozyme (HEWL) in the irradiated area by suppressing convection of the solution using a highly concentrated gel. When a laser pulse, whose energy was above the threshold energy for bubble formation, was focused along with a linear scanning of the stage, HEWL crystallization was enhanced at the surface of bubbles on the irradiated line. The relation between the bubble formation process and the crystallization is discussed. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：6425 / 6429

页数：5

共 20 条

[1] Laser irradiated growth of protein crystal [J].