Cell-free protein synthesis systems

被引：38

作者：

Nakano, H ^{[1
]}

Yamane, T ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Lab Mol Biotechnol, Chikusa Ku, Nagoya, Aichi 46401, Japan

来源：

BIOTECHNOLOGY ADVANCES | 1998年 / 16卷 / 02期

关键词：

protein synthesis; cell-free protein synthesis; cap-independent translation; post-translational modification; cell-free gene translator;

D O I：

10.1016/S0734-9750(97)00082-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell-free protein synthesis systems enable the direct in vitro expression of proteins from template DNA or RNA. Use of biochemical and bioengineering techniques has greatly improved the yields and productivities of cell-free systems. In some cases, the yields approach the in vivo levels. Moreover, in vitro systems are capable of rapidly providing artificial polypeptides that greatly facilitate protein engineering. Post-translational modification steps in cell-free systems also offer exciting possibilities as reviewed here. (C) 1998 Elsevier Science Inc.

引用

页码：367 / 384

页数：18

共 38 条

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