Ultrasensitive Detection of microRNAs by Exponential Isothermal Amplification

被引：473

作者：

Jia, Hongxia ^{[1
]}

Li, Zhengping ^{[1
]}

Liu, Chenghui ^{[1
]}

Cheng, Yongqiang ^{[1
]}

机构：

[1] Hebei Univ, Coll Chem & Environm Sci, Minist Educ, Key Lab Med Chem & Mol Diag, Baoding 071002, Hebei Province, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 32期

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

analytical methods; exponential amplification reaction; fluorescence; microRNA; real-time detection; DNA AMPLIFICATION; MICROARRAY; PROBES; POLYMERASE; EXPRESSION; RNA;

D O I：

10.1002/anie.201001375

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Tiny amounts of a given miRNA (0.1 zmol) can be detected accurately and quantitatively by a real-time method based on an exponential amplification reaction (see picture). The proposed method has a wide dynamic range of more than 10 orders of magnitude, can be carried out within 30 minutes under isothermal conditions, and requires no modified DNA probes. It clearly discriminates miRNA sequences that differ by one base. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

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收藏

页码：5498 / 5501

页数：4

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