Organic-phase enzyme electrode for phenolic determination based on a functionalized sol-gel composite

被引：72

作者：

Wang, BQ

Dong, SJ ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Electroanalyt Chem Lab, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Changchun Inst Appl Chem, Natl Anal & Res Ctr Electrochem & Spect, Changchun 130022, Jilin, Peoples R China

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2000年 / 487卷 / 01期

基金：

中国国家自然科学基金;

关键词：

organic-phase biosensor; tyrosinase; phenolic determination; sol-gel method; hybrid material;

D O I：

10.1016/S0022-0728(00)00152-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric biosensor for monitoring phenols in the organic phase was constructed by the silica sol-gel immobilization of tyrosinase on a glassy carbon electrode. The organic-inorganic hybrid materials with different sol-gel precursors and polymers were optimized, and the experimental conditions, such as the effect of the solvent, operational potential and enzyme loading were explored for the optimum analytical performance of the enzyme electrode. The biosensor can reach 95% of steady-state current in about 18 s, and the trend in the sensitivity of different phenols is as follows: catechol > phenol >p-cresol. In addition, the apparent Michaelis-Menten constants (K-m(app)) and the stability of the enzyme electrode were discussed. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

页码：45 / 50

页数：6

共 41 条

[1] KINETIC CHARACTERIZATION OF THE EFFECTS OF ORGANIC-SOLVENTS ON THE PERFORMANCE OF A PEROXIDASE-MODIFIED ELECTRODE IN DETECTING PEROXIDES, THIOUREA AND ETHYLENETHIOUREA [J].