The CRISPR System: Small RNA-Guided Defense in Bacteria and Archaea

被引：285

作者：

Karginov, Fedor V. ^{[1
,2
]}

Hannon, Gregory J. ^{[1
,2
]}

机构：

[1] Cold Spring Harbor Lab, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA

[2] Cold Spring Harbor Lab, Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA

来源：

MOLECULAR CELL | 2010年 / 37卷 / 01期

基金：

美国国家卫生研究院;

关键词：

COMPLETE GENOME SEQUENCE; MYCOBACTERIUM-TUBERCULOSIS COMPLEX; PROVIDES ACQUIRED-RESISTANCE; SULFOLOBUS-SOLFATARICUS P2; REGULARLY SPACED REPEATS; RAY CRYSTAL-STRUCTURE; STRAIN DIFFERENTIATION; CLOSTRIDIUM-DIFFICILE; ESCHERICHIA-COLI; GENE-TRANSFER;

D O I：

10.1016/j.molcel.2009.12.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

All cellular systems evolve ways to combat predators and genomic parasites. In bacteria and archaea, numerous resistance mechanisms have developed against phage. Our understanding of this defensive repertoire has recently been expanded to include the CRISPR system of clustered, regularly interspaced short palindromic repeats. In this remarkable pathway, short sequence tags from invading genetic elements are actively incorporated into the host's CRISPR locus to be transcribed and processed into a set of small RNAs that guide the destruction of foreign genetic material. Here we review the inner workings of this adaptable and heritable immune system and draw comparisons to small RNA-guided defense mechanisms in eukaryotic cells.

引用

页码：7 / 19

页数：13

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