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Transforming activity of purinergic receptor P2Y, G-protein coupled, 2 revealed by retroviral expression screening

被引：13
作者：
Hatanaka, Hisashi
Takada, Shuji
Choi, Young Lim
Fujiwara, Shin-ichiro
Soda, Manabu
Enomoto, Munehiro
Kurashina, Kentaro
Watanabe, Hideki
Yamashita, Yoshihiro
Sugano, Kentaro
Mano, Hiroyuki
机构：
[1] Jichi Med Univ, Div Funct Genom, Shimotsuke, Tochigi 3290498, Japan
[2] Jichi Med Univ, Div Gastroenterol, Shimotsuke, Tochigi 3290498, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 356卷 / 03期
关键词：
purinergic receptor; oncogene; colorectal carcinoma; cDNA library; retrovirus;
D O I：
10.1016/j.bbrc.2007.03.048
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Colorectal cancer (CRC) is one of the leading causes of cancer death in humans. In order to identify novel cancer-promoting genes in CRC, we here constructed a retroviral cDNA expression library from a CRC cell line RKO, and used it for a focus formation assay with mouse 3T3 fibroblasts, leading to the identification of 42 independent cDNAs. One of such cDNAs turned out to encode purinergic receptor P2Y, G-protein coupled, 2 (P2RY2). The oncogenic potential of P2RY2 was confirmed in vitro with the focus formation assay as well as soft agar-growth assay, and also in vivo with a tumorigenicity assay in nude mice. While our P2RY2 cDNA encodes a protein with two amino-acid substitutions compared to the reported one, we have confirmed that the wild-type P2RY2 has a strong transforming potential as well. These results indicate an unexpected role of P2RY2 in the carcinogenesis of human cancers. (c) 2007 Elsevier Inc. All rights reserved.
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收藏
页码：723 / 726
页数：4
相关论文
共 16 条
[1]   GROWTH-FACTORS AND CANCER [J].
AARONSON, SA .
SCIENCE, 1991, 254 (5035) :1146-1153
[2]   Introduction: P2 receptors [J].
Burnstock, G .
CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2004, 4 (08) :793-803
[3]   Identification of a constitutively active mutant of JAK3 by retroviral expression screening [J].
Choi, Young Lim ;
Kaneda, Ruri ;
Wada, Tomoaki ;
Fujiwara, Shin-ichiro ;
Soda, Manabu ;
Watanabe, Hideki ;
Kurashina, Kentaro ;
Hatanaka, Hisashi ;
Enomoto, Munehiro ;
Takada, Shuji ;
Yamashita, Yoshihiro ;
Mano, Hiroyuki .
LEUKEMIA RESEARCH, 2007, 31 (02) :203-209
[4]   P2X and P2Y purinergic receptors on human intestinal epithelial carcinoma cells: effects of extracellular nucleotides on apoptosis and cell proliferation [J].
Coutinho-Silva, R ;
Stahl, L ;
Cheung, KK ;
de Campos, NE ;
Souza, CD ;
Ojcius, DM ;
Burnstock, G .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2005, 288 (05) :G1024-G1035
[5]   Extracellular nucleotides stimulate proliferation in MCF-7 breast cancer cells via P-2-purinoceptcrs [J].
Dixon, CJ ;
Bowler, WB ;
Fleetwood, P ;
Ginty, AF ;
Gallagher, JA ;
Carron, JA .
BRITISH JOURNAL OF CANCER, 1997, 75 (01) :34-39
[6]   A GENETIC MODEL FOR COLORECTAL TUMORIGENESIS [J].
FEARON, ER ;
VOGELSTEIN, B .
CELL, 1990, 61 (05) :759-767
[7]   Transforming activity of the lymphotoxin-β receptor revealed by expression screening [J].
Fujiwara, S ;
Yamashita, Y ;
Choi, YM ;
Wada, T ;
Kaneda, R ;
Takada, S ;
Maruyama, Y ;
Ozawa, K ;
Mano, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 338 (02) :1256-1262
[8]   Expression of purinergic receptors in non-melanoma skin cancers and their functional roles in A431 cells [J].
Greig, AVH ;
Linge, C ;
Healy, V ;
Lim, P ;
Clayton, E ;
Rustin, MHA ;
McGrouther, DA ;
Burnstock, G .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2003, 121 (02) :315-327
[9]  
Höpfner M, 2001, INT J COLORECTAL DIS, V16, P154
[10]   Cancer statistics, 2006 [J].
Jemal, A ;
Siegel, R ;
Ward, E ;
Murray, T ;
Xu, JQ ;
Smigal, C ;
Thun, MJ .
CA-A CANCER JOURNAL FOR CLINICIANS, 2006, 56 (02) :106-130
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