Detection of attomole amounts of analyte by desorption electrospray ionization mass spectrometry (DESI-MS) determined using fluorescence spectroscopy

被引：44

作者：

Bereman, Michael S. ^{[1
]}

Muddiman, David C. ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Chem, WM Keck FT ICR Mass Spectrometry Lab, Raleigh, NC 27695 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2007年 / 18卷 / 06期

关键词：

D O I：

10.1016/j.jasms.2007.03.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the use of fluorescence spectroscopy to investigate the amount of material removed from a PTFE surface and detected during desorption electrospray ionization (DESI) mass spectrometry measurements. The fluorescence intensity before and after DESI analysis of rhodamine 6G is used to determine the amount of material removed from the surface per mass spectrum. Calculations indicate low attomole amounts are removed per linear ion trap mass spectrum.

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页码：1093 / 1096

页数：4

相关论文

共 21 条

[1] Direct high-resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry [J].

Bereman, Michael S. ;

Nyadong, Leonard ;

Fernandez, Facundo M. ;

Muddiman, David C. .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2006, 20 (22) :3409-3411

[2] Versatile new ion source for the analysis of materials in open air under ambient conditions [J].

Cody, RB ;

Laramée, JA ;

Durst, HD .

ANALYTICAL CHEMISTRY, 2005, 77 (08) :2297-2302

[3] Ambient mass spectrometry [J].

Cooks, RG ;

Ouyang, Z ;

Takats, Z ;

Wiseman, JM .

SCIENCE, 2006, 311 (5767) :1566-1570

[4] ELECTROSPRAY IONIZATION-PRINCIPLES AND PRACTICE [J].

FENN, JB ;

MANN, M ;

MENG, CK ;

WONG, SF ;

WHITEHOUSE, CM .

MASS SPECTROMETRY REVIEWS, 1990, 9 (01) :37-70

[5] ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].

FENN, JB ;

MANN, M ;

MENG, CK ;

WONG, SF ;

WHITEHOUSE, CM .

SCIENCE, 1989, 246 (4926) :64-71

[6] Desorption electrospray ionization using an Orbitrap mass spectrometer: exact mass measurements on drugs and peptides [J].

Hu, Qizhi ;

Talaty, Nari ;

Noll, Robert J. ;

Cooks, R. Graham .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2006, 20 (22) :3403-3408

[7] Direct protein detection from biological media through electrospray-assisted laser desorption ionization/mass spectrometry [J].

Huang, MZ ;

Hsu, HJ ;

Lee, LY ;

Jeng, JY ;

Shiea, JT .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (05) :1107-1116

[8] LASER DESORPTION IONIZATION OF PROTEINS WITH MOLECULAR MASSES EXCEEDING 10000 DALTONS [J].

KARAS, M ;

HILLENKAMP, F .

ANALYTICAL CHEMISTRY, 1988, 60 (20) :2299-2301

[9] Analysis of solids, liquids, and biological tissues using solids probe introduction at atmospheric pressure on commercial LC/MS instruments [J].

McEwen, CN ;

McKay, RG ;

Larsen, BS .

ANALYTICAL CHEMISTRY, 2005, 77 (23) :7826-7831

[10] Direct characterization of intact polypeptides by matrix-assisted laser desorption electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometry [J].

Sampson, Jason S. ;

Hawkridge, Adam M. ;

Muddiman, David C. .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007, 21 (07) :1150-1154