The investigation of single bacteria by means of fluorescence staining and Raman spectroscopy

被引：52

作者：

Krause, M.

Radt, B.

Roesch, P.

Popp, J.

机构：

[1] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

[2] Carl Zeiss, Zent Bereich Forsch & Technol, D-07745 Jena, Germany

[3] Inst Phys Hochtechnol, D-07745 Jena, Germany

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2007年 / 38卷 / 04期

关键词：

Raman spectroscopy; fluorescence staining; microorganisms;

D O I：

10.1002/jrs.1721

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The successful combination of Raman spectroscopy and fluorescence labelling of single bacterial cells is presented. The results show that the selected dyes (DAPI, SYTO 9, CFDA, SYTOX blue and PI) in most cases do not influence the Raman spectrum of a labelled bacterium significantly compared to an unstained bacterium spectrum. The labelling of bacteria offers the possibility of live/dead or biotic/abiotic differentiation prior to a Raman identification step. Copyright (c) 2007 John Wiley & Sons, Ltd.

引用

页码：369 / 372

页数：4

共 8 条

[1] Gene and bacterial identification using high-throughput technologies: Genomics, proteomics, and phonemics [J].