Functionalization of magnetic gold/iron-oxide composite nanoparticles with oligonucleotides and magnetic separation of specific target

被引：33

作者：

Kinoshita, Takuya

Seino, Satoshi

Mizukoshi, Yoshiteru

Nakagawa, Takashi

Yamamoto, Takao A.

机构：

[1] Osaka Univ, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Nagasaki Univ, Fac Engn, Nagasaki 8528521, Japan

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2007年 / 311卷 / 01期

关键词：

gold; iron-oxide; composite nanoparticle; magnetic separation; oligonucleotide;

D O I：

10.1016/j.jmmm.2006.10.1161

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Magnetic composite nanoparticles of gold and iron-oxide synthesized with gamma-rays or ultrasonics were functionalized with thiol-modified oligonucleotides. The amount of oligonucleotides bound to the functionalized nanoparticle probes via hybridization was quantified with fluorescently-labeled target oligonucleotides. Our composite nanoparticles magnetically separated the specific target oligonucleotides without the non-specific adsorption. (c) 2006 Elsevier B.V. All rights reserved.

引用

收藏

页码：255 / 258

页数：4

相关论文

共 15 条

[1] Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology [J].

Daniel, MC ;

Astruc, D .

CHEMICAL REVIEWS, 2004, 104 (01) :293-346

[2] A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles [J].

Demers, LM ;

Mirkin, CA ;

Mucic, RC ;

Reynolds, RA ;

Letsinger, RL ;

Elghanian, R ;

Viswanadham, G .

ANALYTICAL CHEMISTRY, 2000, 72 (22) :5535-5541

[3] Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications [J].

Gupta, AK ;

Gupta, M .

BIOMATERIALS, 2005, 26 (18) :3995-4021

[4] In vitro and in vivo toxicity of magnetic microspheres [J].

Häfeli, UO ;

Pauer, GJ .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 1999, 194 (1-3) :76-82

[5] Magnetic separation of amino acids by gold/iron-oxide composite nanoparticles synthesized by gamma-ray irradiation [J].

Kinoshita, T ;

Seino, S ;

Mizukoshi, Y ;

Otome, Y ;

Nakagawa, T ;

Okitsu, K ;

Yamamoto, TA .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2005, 293 (01) :106-110

[6]

KINOSHITA T, 2005, MAT RES SOC S P E, V877, P1

[7] RETRACTED: A DNA-based method for rationally assembling nanoparticles into macroscopic materials (Retracted article. See vol. 671, 2023) [J].

Mirkin, CA ;

Letsinger, RL ;

Mucic, RC ;

Storhoff, JJ .

NATURE, 1996, 382 (6592) :607-609

[8] Sonochemical preparation of composite nanoparticles of Au/γ-Fe2O3 and magnetic separation of glutathione [J].

Mizukoshi, Y ;

Seino, S ;

Okitsu, K ;

Kinoshita, T ;

Otome, YH ;

Nakagawa, T ;

Yamamoto, TA .

ULTRASONICS SONOCHEMISTRY, 2005, 12 (03) :191-195

[9] DNA-magnetite nanocomposite materials [J].

Mornet, S ;

Vekris, A ;

Bonnet, J ;

Duguet, E ;

Grasset, F ;

Choy, JH ;

Portier, J .

MATERIALS LETTERS, 2000, 42 (03) :183-188

[10] ADSORPTION OF BIFUNCTIONAL ORGANIC DISULFIDES ON GOLD SURFACES [J].

NUZZO, RG ;

ALLARA, DL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (13) :4481-4483