Determination of 1,2,6-inositol trisphosphate (derivatives) in plasma using iron(III)-loaded adsorbents and capillary zone electrophoresis-(indirect) UV detection

被引:9
作者
Buscher, BAP
Tjaden, UR
Irth, H
Andersson, EM
vanderGreef, J
机构
[1] LEIDEN UNIV,LEIDEN AMSTERDAM CTR DRUG RES,DIV ANALYT CHEM,2300 RA LEIDEN,NETHERLANDS
[2] PERSTORP REGENO,S-28480 PERSTORP,SWEDEN
关键词
capillary electrophoresis; adsorbents; sample pretreatment; inositol phosphates;
D O I
10.1016/0021-9673(95)00701-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method for the determination of 1,2,6-inositol trisphosphate (IP3) and derivatives in plasma by capillary zone electrophoresis with (indirect) UV detection has been developed. The sample pretreatment is based on the selective isolation after complexation of inositol phosphates with iron(III) loaded on an adsorbent. Plasma protein denaturation was performed with sodium dodecyl sulfate. The selectivity of the method is demonstrated with the analysis of phenylacetate-IP3. The recoveries amount to 65% and 88% in plasma and in water, respectively.
引用
收藏
页码:413 / 419
页数:7
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