ANALYSIS OF INOSITOL PHOSPHATES AND DERIVATIVES USING CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY

被引：14

作者：

BUSCHER, BAP ^{[1
]}

VANDERHOEVEN, RAM ^{[1
]}

TJADEN, UR ^{[1
]}

ANDERSSON, E ^{[1
]}

VANDERGREEF, J ^{[1
]}

机构：

[1] LEIDEN UNIV,LEIDEN AMSTERDAM CTR DRUG RES,DIV ANALYT CHEM,2300 RA LEIDEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 712卷 / 01期

关键词：

D O I：

10.1016/0021-9673(95)00202-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary zone electrophoresis (CZE) has been combined with mass spectrometric detection for the separation and determination of inositol phosphates (IPs). Apart from IP1 through IP6 (inositol mono- through hexakisphosphate), an IP3 derivative has been analyzed and identified. The detection limits achieved are in the low micromolar range corresponding to an injected amount of ca. 900 fmol. In addition, an IP3 spiked plasma sample was analyzed after sample pretreatment using ultrafiltration.

引用

页码：235 / 243

页数：9

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