FRACTIONATION OF EXTRACTS AND CRYSTALLINE AND NONCRYSTALLINE PROTEINS FROM WHITE KIDNEY BEANS (PHASEOLUS-VULGARIS) BY ION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：1

作者：

ALLI, I

KERMASHA, S

MEHRAN, M

机构：

[1] Dept of Food Science & Agricultural Chemistry, Macdonald College, McGill Univ, Quebec, H9X 1C0

来源：

JOURNAL OF FOOD SCIENCE | 1990年 / 55卷 / 03期

关键词：

D O I：

10.1111/j.1365-2621.1990.tb05228.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Sodium hydroxide solution (0.02%) and citric acid solutions (0.08N, pH 5.5, CA1; 0.60N, pH 3.5, CA2) were used to extract proteins from white kidney beans (Phaseolus vulgaris). The extracts were subjected to ion‐exchange high performance liquid chromatography (IE‐HPLC) using a phosphate elution buffer (20 mM, pH 7.0). The charge properties of the protein fractions present in the NaOH extract and the CA1 and CA2 extracts were similar although the relative proportion of total peak area represented by each fraction was different in the different extracts. IE‐HPLC of the isoelectric precipitate (amorphous microstructure) isolated from the CA1 (bipyramidal crystalline microstructure) and the CA2 (spheroidal microstructure) extracts indicated striking differences in the charge properties of the proteins which constituted the isolates. IE‐HPLC also demonstrated that the precipitation processes did not affect the charge properties of the proteins which remained after the proteins were recovered from the different extracts. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：777 / &

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