SUBSITE DIFFERENCES BETWEEN THE ACTIVE-CENTERS OF PAPAYA PEPTIDASE-A AND PAPAIN AS REVEALED BY AFFINITY-CHROMATOGRAPHY - PURIFICATION OF PAPAYA PEPTIDASE-A BY IONIC-STRENGTH-DEPENDENT AFFINITY ADSORPTION ON AN IMMOBILIZED PEPTIDE INHIBITOR OF PAPAIN

被引：7

作者：

SCHACK, P

KAARSHOLM, NC

机构：

来源：

BIOCHEMICAL JOURNAL | 1984年 / 219卷 / 03期

关键词：

D O I：

10.1042/bj2190727

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：727 / 733

页数：7

共 32 条

[1]

ASBOTH B, 1977, ACTA BIOCHIM BIOPHYS, V12, P223

[2] CHARACTERIZATION OF PAPAYA PEPTIDASE-A AS A CYSTEINE PROTEINASE OF CARICA-PAPAYA L WITH ACTIVE-CENTER PROPERTIES THAT DIFFER FROM THOSE OF PAPAIN BY USING 2,2'-DIPYRIDYL DISULFIDE AND 4-CHLORO-7-NITROBENZOFURAZAN AS REACTIVITY PROBES - USE OF 2-PROTONIC-STATE ELECTROPHILES IN THE IDENTIFICATION OF CATALYTIC-SITE THIOL-GROUPS [J].

BAINES, BS ;

BROCKLEHURST, K .

BIOCHEMICAL JOURNAL, 1982, 205 (01) :205-211

[3] NECESSARY MODIFICATION TO THE PREPARATION OF PAPAIN FROM ANY HIGH-QUALITY LATEX OF CARICA-PAPAYA AND EVIDENCE FOR THE STRUCTURAL INTEGRITY OF THE ENZYME PRODUCED BY TRADITIONAL METHODS [J].

BAINES, BS ;

BROCKLEHURST, K .

BIOCHEMICAL JOURNAL, 1979, 177 (02) :541-548

[4] MAPPING ACTIVE SITE OF PAPAIN WITH AID OF PEPTIDE SUBSTRATES AND INHIBITORS [J].

BERGER, A ;

SCHECHTER, I .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1970, 257 (813) :249-+

[5] PURIFICATION OF PAPAIN BY AFFINITY CHROMATOGRAPHY [J].

BLUMBERG, S ;

SCHECHTER, I ;

BERGER, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 15 (01) :97-+

[6] COVALENT CHROMATOGRAPHY - PREPARATION OF FULLY ACTIVE PAPAIN FROM DRIED PAPAYA-LATEX [J].

BROCKLEHURST, K ;

CARLSSON, J ;

KIERSTAN, MP ;

CROOK, EM .

BIOCHEMICAL JOURNAL, 1973, 133 (03) :573-584

[7] A MARKED GRADATION IN ACTIVE-CENTER PROPERTIES IN THE CYSTEINE PROTEINASES REVEALED BY NEUTRAL AND ANIONIC REACTIVITY PROBES - REACTIVITY CHARACTERISTICS OF THE THIOL-GROUPS OF ACTINIDIN, FICIN, PAPAIN AND PAPAYA PEPTIDASE-A TOWARDS 4,4'-DIPYRIDYL DISULFIDE AND 585'-DITHIOBIS-(2-NITROBENZOATE) DIANION [J].

BROCKLEHURST, K ;

MUSHIRI, SM ;

PATEL, G ;

WILLENBROCK, F .

BIOCHEMICAL JOURNAL, 1983, 209 (03) :873-879

[8] A RE-EVALUATION OF THE NOMENCLATURE OF THE CYSTEINE PROTEINASES OF CARICA-PAPAYA AND A RATIONAL BASIS FOR THEIR IDENTIFICATION [J].

BROCKLEHURST, K ;

SALIH, E .

BIOCHEMICAL JOURNAL, 1983, 213 (02) :559-560

[9] REACTIONS OF PAPAIN AND OF LOW-MOLECULAR-WEIGHT THIOLS WITH SOME AROMATIC DISULFIDES - 2,2'-DIPYRIDYL DISULFIDE AS A CONVENIENT ACTIVE-SITE TITRANT FOR PAPAIN EVEN IN PRESENCE OF OTHER THIOLS [J].

BROCKLEHURST, K ;

LITTLE, G .

BIOCHEMICAL JOURNAL, 1973, 133 (01) :67-80

[10]

Brocklehurst K., 1981, Topics in Enzyme and Fermentation Biotechnology, V5, P262

← 1 2 3 4 →