WATER-SOLUBLE RECEPTORS FOR CYCLIC-AMP AND THEIR USE FOR EVALUATING PHOSPHATE-GUANIDINIUM INTERACTIONS

被引：126

作者：

KATO, Y ^{[1
]}

CONN, MM ^{[1
]}

REBEK, J ^{[1
]}

机构：

[1] MIT,DEPT CHEM,CAMBRIDGE,MA 02139

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1994年 / 116卷 / 08期

关键词：

D O I：

10.1021/ja00087a013

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A water-soluble receptor for adenosine derivatives was synthesized for the study of molecular recognition in aqueous solution. The modular receptor makes use of hydrophobic interactions, Watson-Crick and Hoogsteen hydrogen-bonding, and a phosphate-guanidinium electrostatic interaction to bind cyclic adenosine monophosphates. Measured binding affinities of 2',3/-cAMP are -3.65 and -3.26 kcal/mol at 51 and 501 mM ionic strength, respectively (H2O/D2O solution at 10 degrees C, pH 6.0). The phosphate-guanidinium interaction in this system is estimated to contribute on average 0.6 kcal/mol (51 mM ionic strength) and 0.3 kcal/mol (501 mM ionic strength) to binding. The maximum value of a phosphate-guanidinium electrostatic interaction is estimated to be 2.4 kcal/mol in water.

引用

页码：3279 / 3284

页数：6

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