DIRECT CLONING OF UNMODIFIED PCR PRODUCTS BY EXPLOITING AN ENGINEERED RESTRICTION SITE

被引：11

作者：

TESTORI, A ^{[1
]}

LISTOWSKY, I ^{[1
]}

SOLLITTI, P ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT BIOCHEM,BRONX,NY 10461

来源：

GENE | 1994年 / 143卷 / 01期

关键词：

T VECTORS; OLIGODEOXYRIBONUCLEOTIDES; TAG POLYMERASE; DT-TAILING; ESCHERICHIA COLI;

D O I：

10.1016/0378-1119(94)90623-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A method is described for the direct cloning of DNA fragments amplified by the polymerase chain reaction (PCR). An oligodeoxyribonucleotide, bearing two engineered XcmI sites placed in tandem, was used to generate cloning vectors bearing single 3' deoxythymidine (dT) overhangs at their ends. These 3' dT overhangs are compatible with the 3' deoxyadenosine overhangs found on most Tag polymerase-amplified PCR products. Consequently, Taq polymerase-amplified PCR products can be ligated directly into these modified restriction sites.

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收藏

页码：151 / 152

页数：2

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