DIRECT BRONSTED ANALYSIS OF THE RESTORATION OF ACTIVITY TO A MUTANT ENZYME BY EXOGENOUS AMINES

被引：224

作者：

TONEY, MD ^{[1
]}

KIRSCH, JF ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT BIOCHEM,BERKELEY,CA 94720

来源：

SCIENCE | 1989年 / 243卷 / 4897期

关键词：

D O I：

10.1126/science.2538921

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

引用

页码：1485 / 1488

页数：4

共 22 条

[1] CATALYTIC REACTIONS INVOLVING AZOMETHINES .9. GENERAL BASE CATALYSIS OF TRANSAMINATION OF 3-HYDROXYPYRIDINE-4-ALDEHYDE BY ALANINE [J].

AULD, DS ;

BRUICE, TC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1967, 89 (09) :2098-&

[2] INSIGHTS INTO ENZYME FUNCTION FROM STUDIES ON MUTANTS OF DIHYDROFOLATE-REDUCTASE [J].

BENKOVIC, SJ ;

FIERKE, CA ;

NAYLOR, AM .

SCIENCE, 1988, 239 (4844) :1105-1110

[3] ENGINEERING ENZYME SPECIFICITY BY SUBSTRATE-ASSISTED CATALYSIS [J].

CARTER, P ;

WELLS, JA .

SCIENCE, 1987, 237 (4813) :394-399

[4] STRUCTURAL INVARIANTS IN PROTEIN FOLDING [J].

CHOTHIA, C .

NATURE, 1975, 254 (5498) :304-308

[5]

CHRISTEN P, 1985, TRANSAMINASES, pCH5

[6] COMPUTATION OF MOLECULAR VOLUME [J].

CONNOLLY, ML .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (05) :1118-1124

[7]

Estell D A, 1987, Protein Eng, V1, P441, DOI 10.1093/protein/1.6.441

[8] PROBING STERIC AND HYDROPHOBIC EFFECTS ON ENZYME-SUBSTRATE INTERACTIONS BY PROTEIN ENGINEERING [J].

ESTELL, DA ;

GRAYCAR, TP ;

MILLER, JV ;

POWERS, DB ;

BURNIER, JP ;

NG, PG ;

WELLS, JA .

SCIENCE, 1986, 233 (4764) :659-663

[9] STRUCTURE-ACTIVITY-RELATIONSHIPS IN ENGINEERED PROTEINS - ANALYSIS OF USE OF BINDING-ENERGY BY LINEAR FREE-ENERGY RELATIONSHIPS [J].

FERSHT, AR ;

LEATHERBARROW, RJ ;

WELLS, TNC .

BIOCHEMISTRY, 1987, 26 (19) :6030-6038

[10] QUANTITATIVE-ANALYSIS OF STRUCTURE-ACTIVITY-RELATIONSHIPS IN ENGINEERED PROTEINS BY LINEAR FREE-ENERGY RELATIONSHIPS [J].

FERSHT, AR ;

LEATHERBARROW, RJ ;

WELLS, TNC .

NATURE, 1986, 322 (6076) :284-286

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