A STUDY OF PROCESS INTERACTIONS BETWEEN CELL DISRUPTION AND DEBRIS CLARIFICATION STAGES IN THE RECOVERY OF YEAST INTRACELLULAR PRODUCTS

被引：34

作者：

CLARKSON, AI ^{[1
]}

LEFEVRE, P ^{[1
]}

TITCHENERHOOKER, NJ ^{[1
]}

机构：

[1] RHONE POULENC RORER,RES & DEV,VITRY ALFORTVILLE RES CTR,F-94403 VITRY,FRANCE

来源：

BIOTECHNOLOGY PROGRESS | 1993年 / 9卷 / 05期

关键词：

D O I：

10.1021/bp00023a004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of varying the levels of cell disruption achieved by high-pressure homogenization upon subsequent centrifugal separation of cell debris have been defined by measuring sedimentation velocity distributions of the cell debris. Using this data together with particle size distributions, a computer-based model of a disk stack centrifuge has been used to predict the efficiency of centrifugal separation. These predictions have been compared with both laboratory scale and pilot plant scale experimental results. A similar sedimentation velocity technique has been used to characterize the properties of the flocs formed by addition of poly(ethylene imine) to a borax-clarified homogenate supernatant. The variations of floc settling properties with respect to polymer concentration are defined.

引用

页码：462 / 467

页数：6

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