RAMAN-SPECTROSCOPIC MEASUREMENT OF SPATIAL AND TEMPORAL TEMPERATURE-GRADIENTS IN OPERATING ELECTROPHORESIS CAPILLARIES

被引：85

作者：

LIU, KLK ^{[1
]}

DAVIS, KL ^{[1
]}

MORRIS, MD ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT CHEM,ANN ARBOR,MI 48109

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 21期

关键词：

D O I：

10.1021/ac00093a033

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Steady-state and transient intracapillary temperature gradients are measured by Raman microthermometry during capillary zone electrophoresis. The axial temperature gradient away from a heat sink extends for several millimeters, depending upon the contact of the capillary and heat sink. In free-air convection, small radial gradients, 2-4 degrees C from the center of the lumen to the wall, are observed at 0.85 kW/cm(3). The temperature profile is adequately described by a parabola. With forced cooling, the center-wall temperature difference is less than 1 degrees C. The time to steady-state temperature after power-on is found to be 20 s. The measurements are compared to the results of heat transport calculations.

引用

页码：3744 / 3750

页数：7

共 27 条

[11] INFLUENCE OF THERMAL VARIATION OF DIFFUSION-COEFFICIENT ON NONEQUILIBRIUM PLATE HEIGHT IN CAPILLARY ZONE ELECTROPHORESIS [J].