JUNCTION TRAPPING - A SIMPLE PCR-BASED METHOD FOR THE ISOLATION OF YAC-INSERT TERMINI

被引：3

作者：

PATEL, K

SHEER, D

HAMPTON, GM

机构：

[1] IMPERIAL CANC RES FUND,HUMAN CYTOGENET LAB,LONDON WC2A 3PX,ENGLAND

[2] IMPERIAL CANC RES FUND,CANC GENET LAB,LONDON WC2A 3PX,ENGLAND

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1993年 / 10卷 / 02期

关键词：

D O I：

10.1016/1050-3862(93)90044-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new and potentially reliable method for the isolation of yeast artificial chromosome (YAC)-insert termini, termed junction trapping, is described. This method is based on simple partial digestions of the YAC-containing yeast strain, ligation to a plasmid vector, and selection of the termini by two rounds of polymerase chain reaction (PCR). To date, the method has generated both terminal sequences from each of nine YACs (100%) that have been examined.

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页码：42 / 48

页数：7

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