BATCH FERMENTATIONS WITH A MIXED CULTURE OF LACTIC-ACID BACTERIA IMMOBILIZED SEPARATELY IN KAPPA-CARRAGEENAN LOCUST BEAN GUM GEL BEADS

被引:29
作者
AUDET, P
PAQUIN, C
LACROIX, C
机构
[1] Groupe de recherche STELA, Département de sciences et technologie des aliments, Université Laval, Sainte-Foy, G1K 7P4, Québec
关键词
D O I
10.1007/BF00164736
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Streptococcus salivarius subsp. thermophilus and Lactobacillus delbrueckii subsp. Bulgaricus were immobilized separately in κ-carrageenan-locust bean gum gel beads. The beads were prepared by a dispersion process in a two-phase system (water in oil) and two ranges of bead diameter selected by sieving (0.5-1.0 mm and 1.0-2.0 mm). Fermentations with the two strains were conducted in bench bioreactors in a supplemented whey permeate medium. Free and entrapped cells (two ranges of bead diameter and two levels of initial bead cell load) were grown in mixed culture, and carbohydrate utilization, acid production and cell growth or cell release rate measured. Fermentation rates were influenced by bead diameter and initial cell load of the beads. Beads with high initial cell density increased fermentation rates compared to low cell density beads or free cells. Smaller diameter beads (0.5-1.0 mm) showed a stable tendency (not statistically significant pa > 0.05) towards higher cell release rates, lactose utilization, galactose accumulation and lactic acid production than did larger diameter beads (1.0-2.0 mm). Immobilization of S. salivarius subsp. thermophilus and L. delbrueckii subsp. bulgaricus in separate beads did not seem to affect protocooperation during batch fermentation, and allowed for high cell release rates into the medium. © 1990 Springer-Verlag.
引用
收藏
页码:662 / 668
页数:7
相关论文
共 24 条
  • [11] GILLILAND SE, 1985, BACTERIAL STARTER CU, P41
  • [12] A NEW TECHNIQUE FOR THE PRODUCTION OF IMMOBILIZED BIOCATALYST IN LARGE QUANTITIES
    HULST, AC
    TRAMPER, J
    VANTRIET, K
    WESTERBEEK, JMM
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 1985, 27 (06) : 870 - 876
  • [13] JUILLARD V, 1987, LAIT, V67, P149, DOI 10.1051/lait:1987210
  • [14] KOKOFUTA E, 1988, BIOTECHNOL BIOENG, V32, P756
  • [15] LACROIX C, 1990, IN PRESS APPL MICROB
  • [16] Little T.M., 1978, AGR EXPT DESIGN ANAL
  • [17] MEYER JS, 1975, BIOTECHNOL BIOENG, V27, P1065
  • [18] MONBOUQUETTE HG, 1988, BIOREACTOR IMMOBILIZ, P9
  • [19] COMMENSALISM AND COMPETITION IN MIXED CULTURES OF LACTOBACILLUS-BULGARICUS AND STREPTOCOCCUS-THERMOPHILUS
    MOON, NJ
    REINBOLD, GW
    [J]. JOURNAL OF MILK AND FOOD TECHNOLOGY, 1976, 39 (05): : 337 - 341
  • [20] THE USE OF THE IMMOBILIZATION OF WHOLE LIVING CELLS TO INCREASE STABILITY OF RECOMBINANT PLASMIDS IN ESCHERICHIA-COLI
    NASRI, M
    SAYADI, S
    BARBOTIN, JN
    THOMAS, D
    [J]. JOURNAL OF BIOTECHNOLOGY, 1987, 6 (02) : 147 - 157