COMPARISON OF LIPOSOME IMMUNE LYSIS ASSAY (LILA) AND ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR DETECTION OF ANTIBODIES

被引：5

作者：

TOMIOKA, K

FUKUDA, H

KATOH, S

机构：

[1] KYOTO UNIV,DEPT SYNTHET CHEM & BIOL CHEM,SAKYO KU,KYOTO 60601,JAPAN

[2] KOBE UNIV,DEPT SCI & CHEM ENGN,NADA KU,KOBE,HYOGO 647,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 79卷 / 03期

关键词：

LIPOSOME IMMUNE LYSIS ASSAY (LILA); ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA); SMALL UNILAMELLAR VESICLES (SUV); COMPLEMENT; ALPHA-CHYMOTRYPSINOGEN A;

D O I：

10.1016/0922-338X(95)90618-A

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Anti-alpha-chymotrypsinogen A antibody was assayed by both enzyme-linked immunosorbent assay (ELISA) and liposome immune lysis assay (LILA). The detection limit was slightly affected by the measurement conditions in ELISA; however, it was possible to control the detection limit and to achieve a lower level by adapting the measurement conditions in LILA. LILA is believed to offer a simple and highly sensitive method for measuring the concentration of antibody in serum.

引用

页码：284 / 286

页数：3

共 8 条

[1] A RAPID METHOD FOR DETERMINATION OF THE ASSOCIATION CONSTANT OF MONOCLONAL-ANTIBODIES [J].