IDENTIFICATION OF PHYTATE IN PROTEINS USING POLYACRYLAMIDE DISK GEL-ELECTROPHORESIS

被引：1

作者：

DILOLLO, A ^{[1
]}

ALLI, I ^{[1
]}

KERMASHA, S ^{[1
]}

机构：

[1] MCGILL UNIV,DEPT FOOD SCI & AGR CHEM,MACDONALD CAMPUS,21111 LAKESHORE DR,ST ANNE BELLEVUE H9X 1C0,QUEBEC,CANADA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1991年 / 39卷 / 12期

关键词：

D O I：

10.1021/jf00012a005

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A technique for the identification of protein-phytate complexes on polyacrylamide disc gel electrophoresis precipitates the phytate as a white band of ferric phytate. A chromogenic reaction believed to be specific for iron was evaluated as a means to improve the visualization of the ferric phytate on the gels. The results suggest that white bands, which are formed when protein-phytate complexes on polyacrylamide gels are treated with iron solution, could be the result of nonspecific protein-iron interaction in addition to phytate-iron interaction. These findings were supported by experiments in which proteins containing no phytate gave positive staining for the reaction believed to be specific for phytate-iron complex.

引用

页码：2128 / 2130

页数：3

共 20 条

[1] CONSTITUTION OF LEGUMINOUS SEEDS - SCANNING ELECTRON-MICROSCOPY OF PROTEINS PREPARED FROM PHASEOLUS BEANS [J].