RAPID DETECTION OF SALMONELLA FROM POULTRY MEAT-PRODUCTS USING THE 1.2. TEST

The 1.2 Test® is a new method of Salmonella detection and it was tested in naturally contaminated poultry meat products. The method, first tested on 48 samples using only previous pre‐enrichment phase (experiment 1). gave 30% sensitivity and 91% specificity compared with a classical method. A second study was conducted using the 1.2 Test® following three different cultural procedures ((i) pre‐enrichment, (ii) enrichment, and (iii) a combination of pre‐enrichment and enrichment) (experiment 2). The three cultural procedures, before inoculation of the 1.2 Test®, gave 73, 73, and 93% sensitivity respectively and all of them were 100% specific. Moreover, the 1.2 Test® gave results very similar to those obtained with the ‘enrichment serology’ of Sperber and Deibel. It appears that both the preenrichment and enrichment steps are necessary in order to obtain reliable results from the 1.2 Test®, even with raw and ‘highly’ contaminated products. Copyright © 1990, Wiley Blackwell. All rights reserved