FORMATION OF OLIGOSACCHARIDES FROM LACTOSE BY BACILLUS-CIRCULANS BETA-GALACTOSIDASE

被引：75

作者：

YANAHIRA, S ^{[1
]}

KOBAYASHI, T ^{[1
]}

SUGURI, T ^{[1
]}

NAKAKOSHI, M ^{[1
]}

MIURA, S ^{[1
]}

ISHIKAWA, H ^{[1
]}

NAKAJIMA, I ^{[1
]}

机构：

[1] SNOW BRAND MILK PROD CO LTD, LIFE SCI RES INST, ISHIBASHI, TOCHIGI 32905, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 06期

关键词：

D O I：

10.1271/bbb.59.1021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eleven oligosaccharides formed by a transglycosylation reaction during lactose hydrolysis with Bacillus circulans beta-galactosidase were purified by gel permeation chromatography, charcoal chromatography, and HPLC, From the results of methylation analysis, and MS and NMR studies, it was concluded that these oligosaccharides were beta-D-Galp-(1 --> 3)-D-Glc, beta-D-Galp-(1 --> 6)-D-Glc, beta-D-Galp-(1 --> 2)-D-Glc, beta-D-Galp-(1 --> 4)-beta-D-Galp-(1 --> 4)-D-Glc, beta-D-Galp-(1 --> 6)-[beta-D-Galp-(1 --> 2)]-D-Glc, beta-D-Galp-(1 --> 6)-[beta-D-Galp-(1 --> 4)]-D-Glc, beta-D-Galp-(1 --> 4)-beta-D-Galp-(1 --> 3)-D-Glc, beta-D-Galp-(1 --> 4)-beta-D-Galp-(1 --> 2)-D-Glc, beta-D-Galp-(1 --> 4)-[beta-D-Galp-(1 --> 2)]-D-Glc, beta-D-Galp-(1 --> 4)-beta-D-Galp-(1 --> 6)-D-Glc, beta-D-Galp-(1 --> 6)[beta-D-Galp-(1 --> 3)]-D-Glc. The last five are newly observed oligosaccharides, The results of a use test (in vitro) by human intestinal bacteria showed that the oligosaccharides containing lactose units were predominantly used by human intestinal bifidobacteria.

引用

页码：1021 / 1026

页数：6

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