COMPLEMENTARY USE OF COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN THE SEPARATION OF A SYNTHETIC MIXTURE OF BROMINATED TETRACHLOROFLUORESCEINS

被引：13

作者：

WEISZ, A

SCHER, AL

ANDRZEJEWSKI, D

SHIBUSAWA, Y

ITO, Y

机构：

[1] US FDA,DIV CONTAMINANTS CHEM,WASHINGTON,DC 20204

[2] NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 607卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)87053-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A synthetically prepared mixture of brominated 4,5,6,7-tetrachlorofluoresceins was separated by a combination of preparative reversed-phase high-performance liquid chromatography and high-speed counter-current chromatography. Two new lower-brominated subsidiary colors of D&C Red Nos. 27 and 28 (phloxine B), 4',5'-dibromo-4,5,6,7-tetrachlorofluorescein and 2',4',5'-tribromo-4,5,6,7-tetrachlorofluorescein, were isolated and characterized by H-1 NMR and chemical ionization mass spectrometry.

引用

页码：47 / 53

页数：7

共 8 条

[1] SEMIPREPARATIVE PURIFICATION OF AN ENDOGENOUS LIGAND FOR BRAIN SEROTONIN-2 RECEPTORS BY COIL PLANET CENTRIFUGE COUNTERCURRENT CHROMATOGRAPHY [J].