ALFALFA-STEM PECTINS - ENZYMATIC DEGRADATION AND STRUCTURAL CHARACTERIZATION OF A BUFFER-SOLUBLE FRACTION

被引：12

作者：

HATFIELD, RD ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT AGRON,MADISON,WI 53706

来源：

CARBOHYDRATE RESEARCH | 1991年 / 212卷

关键词：

D O I：

10.1016/0008-6215(91)84054-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbohydrate material solubilized from alfalfa (Medicago sativa L.) stem cell walls with hot 10mM potassium phosphate (pH 7.0) was fractionated into neutral and acidic components by anion-exchange chromatography. Glycosyl-composition and glycosyl-linkage analysis indicated that the neutral fraction contained arabinogalactan type I and that the major acidic fraction was composed of rhamnogalacturonans. The rhamnogalacturonans were resistant to depolymerization by endo-(1 --> 4)-alpha-D-galacturonanase. Saponification of the rhamnogalacturonans followed by acidification to pH 5 gave a precipitate and a soluble fraction. The precipitate after resolubilization was extensively depolymerized by endo-(1 --> 4)-alpha-D-galacturonanase. Rhamnogalacturonans in the soluble fraction, which contained a high proportion of neutral sugar residues, were only partially depolymerized by endo-(1 --> 4)-alpha-D-galacturonanase. Products separated by gel filtration were shown by glycosyl-composition analysis to be composed of different proportions of neutral sugar residues and galactosyluronic residues.

引用

页码：177 / 186

页数：10

共 21 条

[11] AN IMPROVED PROCEDURE FOR THE METHYLATION ANALYSIS OF OLIGOSACCHARIDES AND POLYSACCHARIDES [J].

HARRIS, PJ ;

HENRY, RJ ;

BLAKENEY, AB ;

STONE, BA .

CARBOHYDRATE RESEARCH, 1984, 127 (01) :59-73

[12]

HATFIELD R, 1987, PLANT PHYSL S, V83, P245

[13]

HIRST E. L. et al., 1959, Journal of the Science of Food and Agriculture, V10, P19, DOI 10.1002/jsfa.2740100103

[14]

Lindberg B, 1972, METHODS ENZYMOL, V28, P178

[15] ENZYMATIC AND CHEMICAL DEGRADATION AND THE FINE-STRUCTURE OF PECTINS FROM SUGAR-BEET PULP [J].

ROMBOUTS, FM ;

THIBAULT, JF .

CARBOHYDRATE RESEARCH, 1986, 154 :189-203

[16]

SHEA EM, 1989, PLANT PHYSL S, V89, P850

[17]

STEPHEN AM, 1983, POLYSACCHARIDES, V2, P98

[18]

STEVENS BJH, 1984, PHYTOCHEMISTRY, V23, P107, DOI 10.1016/0031-9422(84)83087-3

[19]

Thibault J. F., 1978, Journal of Food Biochemistry, V2, P379, DOI 10.1111/j.1745-4514.1978.tb00629.x

[20] ENZYMATIC DEGRADATION AND BETA-ELIMINATION OF THE PECTIC SUBSTANCES IN CHERRY FRUITS [J].

THIBAULT, JF .

PHYTOCHEMISTRY, 1983, 22 (07) :1567-1571

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