A PLATE TECHNIQUE FOR SCREENING OF INULIN DEGRADING MICROORGANISMS

被引：20

作者：

CASTRO, GR

BAIGORI, MD

SINERIZ, F

机构：

[1] Cátedra de Microbiología Superior, Facultad de Bioquímica, Química y Farmacia, 4000 Tucumán, Universidad Nacional Tucuman - PROIMI - MIRCEN. Av. Belgrano Pje. Caseros

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1995年 / 22卷 / 01期

关键词：

INULINASE SCREENING; DYE COUPLING; INULIN REMAZOL BRILLIANT BLUE; BACILLUS SP;

D O I：

10.1016/0167-7012(94)00063-D

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A plate assay based on radial diffusion on Petri dishes containing inulin dye labeled with Remazol Brilliant Blue as substrate provides a specific, reliable and rapid inulinase estimation. It is of potential use in the screening of inulin utilizing microorganisms in large numbers of natural samples. In a screening program for inulinolytic sporeformers, 186 isolates were found to degrade inulin as the sole carbon source. Two strains, named VIR 9 and VIR 64, showed high extracellular enzymatic activity compared with a reference strain of Bacillus subtilis MIR 430A.

引用

页码：51 / 56

页数：6

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