N-LINKED SUGAR CHAINS OF 350-KDA ROYAL JELLY GLYCOPROTEIN

被引：41

作者：

KIMURA, Y

WASHINO, N

YONEKURA, M

机构：

[1] API CO LTD, RES INST, GIFU 500, JAPAN

[2] IBARAKI UNIV, SCH AGR, DEPT APPL BIOL RESOURCE SCI, AMI, IBARAKI 30003, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 03期

关键词：

D O I：

10.1271/bbb.59.507

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structures of N-linked sugar chains from 350 kDa royal jelly glycoprotein (RJG), which stimulates the proliferation of human monocytes, have been analyzed. The structural analyses of the N-linked sugar chains released by hydrazinolysis were done by two-dimensional sugar mapping of the corresponding pyridylamino derivatives and 500 MHz H-1-NMR; All four structures were found to fall into the category of oligomannose-type sugar chains as follows; Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->2 Man alpha 1--3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, and Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->3) Man alpha 1-->6(Man alpha 1-->2 Man alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc.

引用

页码：507 / 509

页数：3

共 20 条

[1]

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[2]

FUJIWARA S, 1990, J BIOL CHEM, V265, P11333

[3] IDENTIFICATION METHOD FOR 12 OLIGOMANNOSE-TYPE SUGAR CHAINS THOUGHT TO BE PROCESSING INTERMEDIATES OF GLYCOPROTEINS [J].