ISOLATION AND SEQUENCE-ANALYSIS OF THE CHICKEN GABA(A) RECEPTOR ALPHA-1-SUBUNIT GENE PROMOTER

被引：7

作者：

BATESON, AN

ULTSCH, A

DARLISON, MG

机构：

[1] UNIV ALBERTA,DIV NEUROSCI,EDMONTON,AB T6G 2H7,CANADA

[2] MRC,MOLEC NEUROBIOL UNIT,CAMBRIDGE CB2 2QH,ENGLAND

来源：

GENE | 1995年 / 153卷 / 02期

关键词：

CPG ISLAND; INTRON; TRANSCRIPTIONAL INITIATION; TRANSCRIPTIONAL REGULATION;

D O I：

10.1016/0378-1119(94)00776-O

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A genomic clone containing the 5'- Banking sequence of the chicken GABA(A) receptor alpha 1-subunit-encoding gene (GabR alpha 1) was isolated and characterized. An intron was found to interrupt the 5'-untranslated region. The transcription start point (tsp) was determined by primer extension, RNase protection and the amplification of chick brain first-strand cDNA. DNA sequence analysis revealed a number of putative transcriptional regulatory motifs, including a TATA box 30 nucleotides upstream from the tap, and that this region is a CpG island. While there is conservation between the chicken and human GabR alpha 1 sequences, the chicken GabR alpha 1 promoter has a different structure to those reported fbr the GABA(A) receptor beta 3- and delta-subunit-encoding genes.

引用

页码：243 / 247

页数：5

共 21 条

[1] THE CHICKEN GABA(A) RECEPTOR-ALPHA-1 SUBUNIT - CDNA SEQUENCE AND LOCALIZATION OF THE CORRESPONDING MESSENGER-RNA [J].