SUGAR-CONTAINING LIPIDS PREPARED BY USING A LIPOPHILIC RADICAL INITIATOR - INTERFACIAL RECOGNITION BY LECTIN AS STUDIED BY USING THE MULTIPLE INTERNAL-REFLECTION FLUORESCENCE METHOD

被引：36

作者：

KITANO, H

OHNO, K

机构：

[1] Department of Chemical, Department of Biochemical Engineering, Toyama University

来源：

LANGMUIR | 1994年 / 10卷 / 11期

关键词：

D O I：

10.1021/la00023a036

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A glucose-containing monomer (2-methacryloylolxyethyl alpha-D-glucopyranoside, MEG) was polymerized by using a lipophilic radical initiator. The amphiphile obtained formed a stable liposome by mixing with L-alpha-dipalmitoylphosphatidylcholine (DPPC) or bis(trans,trans-2,4-dioctadecadienoyl)phosphatidylcholine (DDPC, polymerized by UV irradiation after preparation of the liposome), and the interaction of sugar moieties of the amphiphile with a lectin at liquid-lipid and solid-lipid interfaces was investigated by the turbidimetry and the multiple internal reflection fluorescence method, respectively. The rate of binding of the sugar-containing liposome to the surface modified with the lectin was smaller than the theoretical value for a simple collision and strongly dependent on both weight percent of the sugar lipid in the liposome and the degree of polymerization of MEG.

引用

页码：4131 / 4135

页数：5

共 16 条

[11]

Macritchie F, 1978, Adv Protein Chem, V32, P283, DOI 10.1016/S0065-3233(08)60577-X

[12] BINDING-KINETICS OF ANTIBODIES TO ANTIGENS ON POLYMER SURFACES AS STUDIED BY THE MULTIPLE INTERNAL-REFLECTION FLUORESCENCE METHOD [J].