AN AMPEROMETRIC OPIATE ASSAY

被引：23

作者：

HOLT, PJ ^{[1
]}

STEPHENS, G ^{[1
]}

BRUCE, NC ^{[1
]}

LOWE, CR ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,INST BIOTECHNOL,CAMBRIDGE CB2 1QT,ENGLAND

来源：

BIOSENSORS & BIOELECTRONICS | 1995年 / 10卷 / 6-7期

关键词：

HEROIN; MORPHINE; HEROIN ESTERASE; MORPHINE DEHYDROGENASE; AMPEROMETRIC ASSAY; MEDIATOR; PHENAZINE METHOSULFATE;

D O I：

10.1016/0956-5663(95)96927-Q

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Current techniques for the detection and measurement of diacetylmorphine (heroin), morphine and their principal metabolite morphine-3-glucuronide (M3G) are based mainly on chromatography or immunoassay. Na enzymatic method for the detection of these compounds has yet been reported. Two novel microbial enzymes have been isolated and characterized in this laboratory: an acetylmorphine carboxyesterase (heroin esterase) and a morphine dehydrogenase (MDH). These highly specific enzymes have been incorporated in an amperometric assay for heroin and morphine using phenazine methosulphate as a mediator. The assay gives a rapid and sensitive response to heroin and morphine, with a detection limit for morphine of 6.8 mu g ml(-1) (23.7 mu M).

引用

页码：517 / 526

页数：10

共 37 条

[21]

GUILBAULT GG, 1991, BIOTECHNOL APPL BIOC, V14, P133

[22]

Hall GE, 1937, J PHARMACOL EXP THER, V61, P10

[23] DETERMINATION OF PHOLCODINE AND ITS METABOLITES IN URINE BY CAPILLARY GAS-CHROMATOGRAPHY [J].

JOHANSEN, M ;

RASMUSSEN, KE ;

CHRISTOPHERSEN, AS .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 532 (02) :277-284

[24]

KUMAGAI Y, 1990, J PHARMACOL EXP THER, V255, P504

[25]

KUPFERBERG H, 1964, J PHARMACOL EXP THER, V145, P547

[26] SYSTEMATIC IDENTIFICATION OF DRUGS OF ABUSE .1. SPOT TESTS [J].

MASOUD, AN .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1975, 64 (05) :841-844

[27]

MOFFAT AC, 1986, CLARKES ISOLATION ID

[28] SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SEPARATION OF 3,6-DIACETYLMORPHINE HYDROCHLORIDE (HEROIN) AND HYDROLYSIS PRODUCTS [J].

POOCHIKIAN, GK ;

CRADOCK, JC .

JOURNAL OF CHROMATOGRAPHY, 1979, 171 (APR) :371-376

[29] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF MORPHINE IN BIOLOGICAL SAMPLES - AN OVERVIEW OF SEPARATION METHODS AND DETECTION TECHNIQUES [J].

TAGLIARO, F ;

FRANCHI, D ;

DORIZZI, R ;

MARIGO, M .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 488 (01) :215-228

[30]

TRUTKO SM, 1988, MICROBIOLOGY+, V57, P763

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