EVALUATION OF HISTIDINE-DECARBOXYLASE ACTIVITY OF BACTERIA ISOLATED FROM SARDINE (SARDINA-PILCHARDUS) BY AN ENZYMATIC METHOD

被引：53

作者：

LOPEZSABATER, EI

RODRIGUEZJEREZ, JJ

HERNANDEZHERRERO, M

MORAVENTURA, MT

机构：

[1] Department of Food Hygiene. Faculty of Veterinary Medicine, Universidad Autónoma de Barcelona (U.A.B.), Barcelona

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 19卷 / 02期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00908.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A fast and simple enzymic method has been adapted to measure histidine decarboxylase activity in bacteria isolated from fish. It was possible to quantify the presence of histamine at levels higher than 1.03 x 10(-2) mu mol (3 ppm) per mi of culture medium. There was a good correlation (r = 0.99) between the histamine content and the increase of absorbance in the concentration range of 3 and 30 ppm. The method can be used for quantitation of the amount of histamine produced by a selected strain, and has the advantages of being low in cost, reliable, easy to use and fast to perform. Plesiomonas shigelloides, a bacterium frequently isolated from fish and aquatic environments, has been identified as a new histamine-former in fish.

引用

页码：70 / 75

页数：6

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